Sequencing of the whole mitochondrial genome of the. In the pollen loads, we identified 40 pollen types. Apidologie long distance foraging and recruitment by a stingless bee, melipona mandacaia brunno kuhn felipe a. In northeastern brazil, especially in the semiarid region, animals and plants are widely used in traditional medicine and play significant roles in healing practices. Melipona print iconographia zoologica special collections university of amsterdam ubainv0274 045 10 0036. We analyzed genetic variability and populational structure of melipona scutellaris, based on five microsatellite loci, using heterologous primers of m. Since the mitochondrial genome is relatively short, it has been proved that long pcr products, obtained from high fidelity enzymes, are effective to cover the whole mitochondrial genome in metazoa species. Melipona scutellaris obtained second place in visits with rr 23. The two seasons dry and rainy presented high similarity morisita index 0. In vitro larval rearing protocol for the stingless bee species melipona scutellaris for toxicological studies. Gastroprotective effect of geopropolis from melipona. The gastroprotective activity of the eegp was evaluated using model ulcer induced by ethanol. With advances in molecular biology, such visual criteria proved not to be reliable.
Here we analyzed those hypotheses comparing the use of floral sources of pollen, through paired analyses of. Pdf in vitro larval rearing protocol for the stingless. In silico analysis of a set of cdna library sequences yielded 1728 expressed sequence tags ests and 997 highquality sequences that were assembled into 29 contigs and 117 singlets. Here we describe the discovery of two novel cyclic hexadepsipeptides, meliponamycin a 1 and meliponamycin b 2. The experiment was carried out by applying fungus suspension on the back and by contact on recently emerged bees. The samples were directly collected from the pollen pots of t. Melipona scutellaris melipona scutellaris melipona scutellaris 21. Sequencing of the whole mitochondrial genome of the brazilian stingless bee. Pollen types present in samples from corbiculae of melipona capixaba moure and camargo hymenoptera. In addition to the physical barrier, the epidermis also presents an innate defense against microbial overgrowth. Generalist foraging behavior among stingless bees is accepted but untested, as well as the subsidiary hypothesis of floral preferences in the genus melipona. Metals in geopropolis from beehive of melipona scutellaris. At first, the characterization of bees was based mostly on morphological and behavioral criteria. Animalbased remedies as complementary medicines in santa.
Scutellaris colony 349 material and methods a brood disk from the m. Differentially displayed expressed sequence tags in melipona scutellaris hymenoptera, apidae, meliponini development. Mead is a traditional alcoholic beverage obtained by fermenting must and can offer a solution to honey over. Dec 15, 2014 melipona scutellaris urucu cafe maca goiaba abacate pitanga pitomba m. Research article bioactive fraction of geopropolis from. It is considered to be the reared melipona species with the largest distribution in the north and northeast regions of brazil, with records from rio grande do norte down to bahia. Failure to find proboscis conditioning in oneday old. Managing and preserving stingless bees springerlink. Insights into the melipona scutellaris hymenoptera.
Effect of propolis oral intake on physiological condition of. The sequences of pcr products and ests, together covered 28% of the reference mitochondrial genome. Melipona scutellaris geopropolis antimicrobial activity antiproliferative activity chemical profile electronic supplementary material the online version of this article doi. In this same phase a correlation also exists between the number of cells and the volume of the corpora allata r 0. Cloning and expressing a highly functional and substrate speci. One important factor is the influence of nestmates. Insights into the melipona scutellaris hymenoptera, apidae, meliponini fat body transcriptome. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs. An updating bibliography of the bees of the worldbook a. Pdf differentially displayed expressed sequence tags in. Apr 23, 2010 strict regulation of caste differentiation, at the molecular level, is thought to be important to maintain social structure in insect societies. Floral odours brought back to the colony by successful foragers are important sources of information underlying an individuals decision about which resource to collect. Use of polliniferous resources by melipona capixaba, an.
All insect defensins belonging to the invertebrate class have a consensus motif, cx516cx3cx910cx47cx1c. Here you can download the conference program and complete proceedings as a single pdf, or search for particular abstracts by symposium or author. Biology of pollination of citrus sinensis variety pera. Susceptibility of melipona scutellaris latreille, 1811.
This is the first palynological study using pollen stored by tetragonisca angustula apidae. The objective of the present study was to assess the susceptibility of melipona scutellaris bees to the isolate of the entomopathogenic fungus beauveria bassiana. So, the aim of the present study was to report heat production by nurse bees of the stingless bee melipona scutellaris working on the brood combs. Abelhas urucu melipona scutellaris crias 8349462207. This is a pdf file of an unedited manuscript that has been accepted for publication. Whether youve loved the book or not, if you give your honest and detailed thoughts then people will find new books that are right for them.
The important role of pollinators such as the stingless bee melipona scutellaris in nature encouraged us to pursue efforts to study its associated microbiota. Defensins are a well known family of cationic antibacterial peptides amps isolated from fungi, plants, insects, mussels, birds, and various mammals. Identification, phylogenetic analysis and expression profile. However, studies concerning the biological activity and chemical composition of geopropolis are scarce.
Melipona is a genus of stingless bees, widespread in warm areas of the neotropics, from sinaloa and tamaulipas mexico to tucuman and misiones argentina. Geopropolis is a kind of propolis composed by resin, wax, and soil collected by bee and. Determination and uptake of abamectin and difenoconazole in the stingless bee melipona scutellaris latreille. Previously, a number of extrinsic and intrinsic factors have been shown to influence caste composition in termite colonies. Other readers will always be interested in your opinion of the books youve read. Social insects establish complex interactions with microorganisms, some of which play defensive roles in colony protection. Authorsofpaper 2014 titleofabstract in 17th congress of the international union for the study of social insects iussi, cairns, australia, 18 july 2014. Additional experiments examined the suitability of.
Pollen types from colonies of melipona scutellaris. Apis mellifera, melipona scutellaris, stingless bees, pollen, brazil abstract. Media in category melipona scutellaris the following 8 files are in this category, out of 8 total. For melipona scutellaris, we observed that virgin queens were executed when they were between 5 and 9 days old, while newly emerged queens were not attacked. Influence of application rates of beecollected pollen on the fruit size of kiwi. Cloning and expressing a highly functional and substrate. To date, microsatellite primers have been developed for eight species of stingless bees.
We analysed 117 pollen loads and pollen content of 15 honey samples. Research article apolar bioactive fraction of melipona scutellaris. Melipona quadrifasciata is a species of eusocial, stingless bee of the order hymenoptera. Using illumina miseq data, we achieved the annotation of all protein coding genes, as well as the genes for the two ribosomal subunits 16s and 12s. Determination and uptake of abamectin and difenoconazole in. Antibiotics free fulltext antibiotic delivery strategies. Origin of geopropolis samples from melipona scutellaris hymenoptera. The faster movements of old virgin in relation to newly emerged might be responsible for attacks. Geopropolis is a type of propolis containing resin, wax, and soil, collected by threatened stingless bee species native to tropical countries and used in folk medicine. Long distance foraging and recruitment by a stingless bee. Offspring analysis in a polygyne colony of melipona. Circulating hemocytes from larvae of melipona scutellaris hymenoptera, apidae, meliponini. Melipona scutellaris and at investigating the possible mechanisms of action.
Study of the number of chromosomes and of spermatozoa in the stingless bee melipona scutellaris, latreille, 1811. Determination and uptake of abamectin and difenoconazole in the stingless bee melipona scutellaris. Mar 01, 2012 the social organization of foraging processes is a principal key to the ecological success of eusocial insects. Recommended articles citing articles 0 references anvisa, 2019a. If you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs. In particular, we provide genetic evidence which shows that, upon loss of the mother queen, many colonies are invaded by unrelated queens that fly in from unrelated hives nearby. Please contribute to wikispecies by verifing the validity of this taxon and adding the appropriate references. Determination and uptake of abamectin and difenoconazole. Here, we present a novel example of an intraspecific social parasitism in a highly eusocial bee, the stingless bee melipona scutellaris.
Melipona scutellaris is a eusocial stingless bee species of the order hymenoptera and the genus melipona. Preliminary characterization of mitochondrial genome of. However, in the crucial phase for caste determination in melipona, that is prepupae2, a strong positive corre lation was found r 0. New penicillium and talaromyces species from honey, pollen. Jan 28, 20 geopropolis is a type of propolis containing resin, wax, and soil, collected by threatened stingless bee species native to tropical countries and used in folk medicine. Pages in category melipona the following 5 pages are in this category, out of 5 total. Zootherapies form an integral part of these cultures, and information about animals is passed from generation to generation through oral.
Foodsourcenaive individuals memorize and use this olfactory information when searching for food in the field. Therefore, we propose next, using specific primers of m. Antimicrobial and antiproliferative activities of stingless. The epidermal skin barrier protects the body from a host of daily challenges, providing protection against mechanical insults and the absorption of chemicals and xenobiotics. Research article apolar bioactive fraction of melipona.
Determination of acute lethal doses ld50 and lc50 of. Article pdf available in iheringia serie zoologia 10112 june 2011 with 32 reads. Palynological examination of the pollen pots of native. Experiments were designed to investigate classical conditioning, pseudoconditioning,and central excitatory state. Pdf sequencing of the whole mitochondrial genome of the. Clinical signs of brood disease brood rot were found in meliponini colonies of the meliponary stingless bee colonies distributed in an open and roofed area, in an orchard, fig. The aim of this study was to evaluate the gastroprotective activity of ethanolic extract of geopropolis eegp from melipona scutellaris and to investigate the possible mechanisms of action. Nov 12, 2016 in queenright colonies of stingless bees of the genus melipona, workers recognize, attack, and kill young virgin queens. Here we present the assembly, annotation, and characterization of a draft mitochondrial genome of the brazilian stingless bee melipona scutellaris using melipona bicolor as a reference organism. Research article gastroprotective effect of geopropolis. The proboscis extension reflex was used to investigate behaviormodification in one day old africanized honey bees and in adult uruu honey bees.
Presentation mode open print download current view. The aim of this study was to evaluate the activity of the ethanolic extract of geopropolis eegp from melipona scutellaris and its fractions on the modulation of neutrophil migration in the inflammatory process, and the participation of nitric oxide no pathway, as well as to check the chemical profile of the bioactive fraction. Pdf does beekeeping reduce genetic variability in melipona. Pdf insights into the melipona scutellaris hymenoptera. The purpose of this study was to produce and characterize mead with different levels of sugars and alcohol obtained from honey from melipona scutellaris.
It stands out due to its highquality honey and for being responsible for 40 to 90% of the pollination of native plant species. Population divergence of melipona scutellaris hymenoptera. The largest producer of honey from melipona bees in mexico is in the state of yucatan where bees are studied at an interactive park called bee planet. In this study, we analyzed the transcriptome of the fat body from the stingless bee melipona scutellaris. To elucidate the possible mechanisms of action, we investigated the involvement of the nonprotein sulfhydryl. Sequencing of the whole mitochondrial genome of the brazilian. Jul 22, 2008 the use of animal products in healing is an ancient and widespread crosscultural practice. Apidae from seven meliponaries m located in and outside of an urbanindustrial region, state of bahia, brazil.
Os orificios tambem auxiliam na ventilacao da colonia. This study aimed to identify pollen types used by melipona scutellaris through the analysis of pollen loads and honey samples in colonies established in a coffee plantation. In this study, we evaluated the antimicrobial and antiproliferative activity of the ethanolic extract of geopropolis eegp. In particular, we provide genetic evidence which shows that, upon loss of the mother queen, many colonies are invaded by unrelated queens. Melipona scutellaris, a brazilian stingless bee, commonly found in the northeast region 2.
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